6XBC

Crystal structure of Streptomyces sviceus SsDesB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	83.046, 151.174, 141.401
Unit cell angles	90.00, 91.58, 90.00

Refinement procedure

Resolution	39.119 - 2.863
R-factor	0.202
Rwork	0.199
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o8r
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.860	2.860
Rmerge	0.043	0.355
Rmeas	0.079	0.537
Rpim	0.042	0.288
Number of reflections	78957	3917
<I/σ(I)>	2.2	2.2
Completeness [%]	98.7
Redundancy	3.2
CC(1/2)	0.996	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M HEPES pH 7.5, 0.2M NaCl, 20% w/v PEG3350, 10 mM taurine