6XAT

Crystal structure of the human FoxP4 DNA binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45870
Spacegroup name	C 2 2 21
Unit cell lengths	40.449, 70.648, 63.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.100 - 2.200
R-factor	0.1995
Rwork	0.198
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a07
RMSD bond length	0.011
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.100	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.023	0.332
Rmeas	0.032	0.469
Rpim	0.023	0.374
Number of reflections	4862	488
<I/σ(I)>	19.7	2.17
Completeness [%]	99.9	100
Redundancy	12.4
CC(1/2)	1.000	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	Sodium Formate 4 M