6XAR

Structure of CBL tyrosine kinase binding domain (TKBD) with C-terminal tail of Src-like kinase protein 2 (SLAP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	62.730, 86.960, 65.260
Unit cell angles	90.00, 112.31, 90.00

Refinement procedure

Resolution	45.323 - 2.501
R-factor	0.2515
Rwork	0.249
R-free	0.29340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b47
RMSD bond length	0.002
RMSD bond angle	0.602
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (dev)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.323	45.320	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.080	0.039	0.601
Rmeas	0.098	0.047	0.806
Rpim	0.055	0.027	0.530
Total number of observations		1340	2833
Number of reflections	20374	486	1447
<I/σ(I)>	8.3	18.3	1.5
Completeness [%]	90.5	96.2	57.8
Redundancy	2.9	2.8	2
CC(1/2)	0.993	0.995	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	CBL/SLAP2 complex mixed in hanging drop with equal volume (200 nL) of 0.1 M Hepes pH 7.5, 10% (w/v) PEG8000