6X7V

Crystal Structure of the Human Nudix Hydrolase Nudt16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	85.686, 137.922, 54.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.280 - 2.300
R-factor	0.2275
Rwork	0.224
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mgm
Data reduction software	XDS (20180808)
Data scaling software	XDS (20180808)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.765
Number of reflections	28854	4458
<I/σ(I)>	10.5
Completeness [%]	97.6	95.3
Redundancy	4.1	4.2
CC(1/2)	0.998	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M ammonium chloride, 20% (w/v) PEG 3350, 0.1 M MES pH 5.5