6X76

Rev1 L325G Mn2+-facilitated Product Complex with second dCTP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-02
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	61.965, 73.626, 117.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.130 - 2.530
R-factor	0.2096
Rwork	0.203
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wm1
RMSD bond length	0.010
RMSD bond angle	1.206
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.130	3.070
High resolution limit [Å]	2.530	2.530
Rmeas	0.165
Number of reflections	31942	12900
<I/σ(I)>	7.8
Completeness [%]	99.4
Redundancy	3.8
CC(1/2)		0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15-23% PEG3350, 200 mM ammonium nitrate