6X75

Rev1 Mn2+-facilitated Product Complex with second dCTP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-04
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.199, 73.566, 117.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.690 - 1.950
R-factor	0.1857
Rwork	0.183
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wm1
RMSD bond length	0.008
RMSD bond angle	0.925
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.690	2.492
High resolution limit [Å]	1.950	1.980
Rmeas	0.163
Number of reflections	63991	27855
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	7.9
CC(1/2)		0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15-23% PEG3350 and 200 mM ammonium nitrate