6X74

Rev1 Mg2+-facilitated Product Complex with no monophosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-19
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.384, 72.436, 118.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.540 - 1.690
R-factor	0.197
Rwork	0.196
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wm1
RMSD bond length	0.008
RMSD bond angle	0.936
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.540	1.847
High resolution limit [Å]	1.690	1.700
Rmeas	0.091
Number of reflections	113949	25198
<I/σ(I)>	27.5
Completeness [%]	99.9
Redundancy	9
CC(1/2)		0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15-23% PEG3350 and 200 mM ammonium nitrate