6X70
Rev1-DNA Binary Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-24 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.212, 73.916, 117.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.490 - 2.050 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wm1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.407 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.147 | 0.473 |
| Number of reflections | 60116 | 24230 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.6 | 97 |
| Redundancy | 3.1 | 2.9 |
| CC(1/2) | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15-23% PEG3350 and 200 mM ammonium nitrate |
