6X6Z

Rev1 Ternary Complex with dCTP and Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-18
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	62.376, 73.947, 117.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.260 - 1.400
R-factor	0.1869
Rwork	0.186
R-free	0.20740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wm1
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.260	1.600
High resolution limit [Å]	1.400	1.400
Rmeas	0.070
Number of reflections	198840	63131
<I/σ(I)>	25.4
Completeness [%]	99.5
Redundancy	4
CC(1/2)		0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15-23% PEG3350 and 200 mM ammonium nitrate