6X6F

The structure of Pf6r from the filamentous phage Pf6 of Pseudomonas aeruginosa PA01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.880, 160.310, 45.670
Unit cell angles	90.00, 116.28, 90.00

Refinement procedure

Resolution	40.950 - 1.735
R-factor	0.1736
Rwork	0.173
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wnm
RMSD bond length	0.005
RMSD bond angle	0.854
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.950	1.797
High resolution limit [Å]	1.735	1.735
Rmerge	0.062	0.527
Rmeas	0.087	0.742
Rpim	0.061	0.522
Number of reflections	59270	5818
<I/σ(I)>	12.3	2
Completeness [%]	99.5	97.52
Redundancy	3.8
CC(1/2)	0.997	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	20% Polyethylene glycol mono-methyl ether 5000, 200 mM Ammonium nitrate 10 mM HEPES, ph 7.5, 150 mM NaCl, 5% glycerol, 1 mM TCEP. 25% PEG 200 final cryoprotectant