6X60

ClpP2 from Chlamydia trachomatis with resolved handle loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-10-31
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	129.710, 147.043, 97.059
Unit cell angles	90.00, 128.25, 90.00

Refinement procedure

Resolution	28.160 - 2.810
R-factor	0.2003
Rwork	0.198
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jct
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc2_3794)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmeas	0.096	0.712
Rpim	0.049	0.374
Number of reflections	34843	2318
<I/σ(I)>	15.25	2.07
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)		0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	Tacsimate pH 7