6X5R

Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A2-Asn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-08
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 65 2 2
Unit cell lengths	149.880, 149.880, 480.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.409 - 2.400
R-factor	0.2067
Rwork	0.205
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2de0
RMSD bond length	0.010
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.409	2.460
High resolution limit [Å]	2.400	2.400
Rmeas	0.153	1.500
Number of reflections	124545	8849
<I/σ(I)>	18.4	1.4
Completeness [%]	99.3	97.1
Redundancy	10.4	11.7
CC(1/2)	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.0 M Lithium sulfate, 10 mM Nickel chloride, 100 mM Tris pH 8.5