6X5N
Crystal structure of a stabilized PAN ENE bimolecular triplex with a GC-clamped polyA tail, in complex with Fab-BL-3,6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.299, 76.182, 110.523 |
| Unit cell angles | 72.22, 88.58, 86.57 |
Refinement procedure
| Resolution | 37.990 - 3.300 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b14 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.336 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.430 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.108 | |
| Number of reflections | 18323 | 3255 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 90.8 | |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris HCL, 0.2 M Li2SO4, 22% PEG 3350 |
