6X5M
Crystal structure of a stabilized PAN ENE bimolecular triplex with a GC-clamped polyA tail, in complex with Fab-BL-3,6.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.128, 76.260, 110.664 |
| Unit cell angles | 71.90, 88.59, 86.58 |
Refinement procedure
| Resolution | 38.040 - 2.500 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b14 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.380 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 | 2.015 |
| Number of reflections | 41850 | 3417 |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 90.4 | 70.1 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris HCL, 0.2 M Li2SO4, 22% PEG 3350 |
