6X58

MPER-Fluc-Ec2 bound to 10E8v4 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976
Spacegroup name	P 32
Unit cell lengths	99.050, 99.050, 167.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.020 - 3.260
R-factor	0.2533
Rwork	0.251
R-free	0.29510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a43
Data reduction software	xia2
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	REFMAC (1.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.180	38.180	3.460
High resolution limit [Å]	3.260	9.780	3.260
Rmerge	0.135	0.044	2.441
Rmeas	0.141	0.046	2.558
Rpim	0.043	0.014	0.763
Total number of observations	306181	10949	51725
Number of reflections	28368	1019	4601
<I/σ(I)>	10.3	35.3	1
Completeness [%]	98.6	95.1	98.4
Redundancy	10.8	10.7	11.2
CC(1/2)	0.999	0.999	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	34-39% PEG 300, 0.1 M NaCl, 0.1 M MES pH 6.2-7