6X4H
Sortilin-Progranulin Interaction With Compound 24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.458, 79.444, 111.545 |
| Unit cell angles | 90.00, 127.00, 90.00 |
Refinement procedure
| Resolution | 32.290 - 2.900 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.000 | 2.913 |
| High resolution limit [Å] | 2.900 | 2.904 |
| Rmerge | 0.054 | 0.522 |
| Number of reflections | 24571 | 231 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 97.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG3350, 0.4M Na Malonate, pH8, 0.1M Tris, 5% Glycerol |
