6X48
Sortilin-Progranulin Interaction With Compound 17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-07 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.350, 78.490, 111.220 |
| Unit cell angles | 90.00, 127.23, 90.00 |
Refinement procedure
| Resolution | 64.630 - 2.900 |
| R-factor | 0.1963 |
| Rwork | 0.193 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.630 | 2.913 |
| High resolution limit [Å] | 2.900 | 2.904 |
| Rmerge | 0.070 | 0.592 |
| Number of reflections | 24183 | 252 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG3350, 0.4M Na Malonate, pH8, 0.1M Tris, 5% Glycerol |
