6X3M

Crystal structure of full-length Streptococcal bacteriophage hyaluronidase in complex with unsaturated hyaluronan octa-saccharides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	338.168, 56.797, 276.954
Unit cell angles	90.00, 126.68, 90.00

Refinement procedure

Resolution	29.867 - 3.581
R-factor	0.25
Rwork	0.247
R-free	0.29910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c3f 1bkv
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.730
High resolution limit [Å]	3.581	3.600
Rmerge	0.124	0.383
Number of reflections	49298	4295
<I/σ(I)>	6.5	2.52
Completeness [%]	97.5	86.5
Redundancy	4.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	17.5-18.5% (w/v) PEG 2000 MME, 70 mM KBr, 62.5 mM Glycine, 100 mM Tris-HCl, pH 8.5; prior to crystallization the protein was incubated with unsaturated hyaluronan octa-saccharides at a molar ratio of 1:50