6X08

Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 64 2 2
Unit cell lengths	233.992, 233.992, 139.424
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	117.000 - 4.190
R-factor	0.3131
Rwork	0.311
R-free	0.34650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ewe
RMSD bond length	0.006
RMSD bond angle	0.949
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.000	4.350
High resolution limit [Å]	4.190	4.190
Rpim	0.056	0.665
Number of reflections	17026	1665
<I/σ(I)>	19.1	1.32
Completeness [%]	100.0
Redundancy	37.9
CC(1/2)	0.982	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1M ammonium sulfate, di-sodium succinate pH 5.5, and the addition of 4% 1-propanol