6X06

Nup120 (aa1-757) from S. cerevisiae bound by VHH-SAN11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	193.170, 193.170, 78.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.650 - 4.270
R-factor	0.3364
Rwork	0.334
R-free	0.35930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hxr
RMSD bond length	0.002
RMSD bond angle	0.605
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.650	4.400
High resolution limit [Å]	4.250	4.250
Rpim	0.052	0.512
Number of reflections	11664	1141
<I/σ(I)>	18.7	1.37
Completeness [%]	99.5
Redundancy	7.3	7.2
CC(1/2)	0.938	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	19% PEG 8,000, 0.1M magnesium chloride, 0.1M Tris/HCl pH 8.5