6WZY
Structure of DbNA(10) peptides bound to H-2Db MHC-I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.722, 44.969, 71.774 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 37.510 - 1.500 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ftg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.028 | 0.390 |
| Number of reflections | 69128 | 3413 |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 99.1 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 0.1M sodium citrate pH 5.7, 0.2M LiSO4 and 24-28% (w/v) PEG3350 |
