6WZO
Structure of SARS-CoV-2 Nucleocapsid dimerization domain, P1 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 |
Unit cell lengths | 43.718, 50.061, 69.337 |
Unit cell angles | 106.50, 90.09, 97.14 |
Refinement procedure
Resolution | 66.430 - 1.420 |
R-factor | 0.1581 |
Rwork | 0.157 |
R-free | 0.17320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gib |
RMSD bond length | 0.007 |
RMSD bond angle | 0.888 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.430 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.310 | |
Rmeas | 0.046 | 0.363 |
Number of reflections | 96446 | 3939 |
<I/σ(I)> | 16.8 | 3 |
Completeness [%] | 91.1 | |
Redundancy | 3.7 | |
CC(1/2) | 0.999 | 0.957 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Sodium acetate pH 4.5, 50 mM Sodium/potassium tartrate, and 34% polyethylene glycol PEG 3350 |