6WZA

Ni-bound structure of an engineered metal-dependent protein trimer, TriCyt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.239840
Spacegroup name	P 31 2 1
Unit cell lengths	82.115, 82.115, 137.517
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.346 - 2.500
R-factor	0.2473
Rwork	0.241
R-free	0.30590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bc5
RMSD bond length	0.009
RMSD bond angle	1.058
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.346	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.142	0.688
Rmeas	0.145	0.706
Rpim	0.033	0.159
Number of reflections	19138	1865
<I/σ(I)>	18.23
Completeness [%]	99.7
Redundancy	19.2
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	22.5% PEG 400, 0.2 M NaCl, 0.1 M HEPES (pH 7.5)