6WYT

Crystal structure of anti-Muscle Specific Kinase (MuSK) Fab, MuSK1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	62.420, 66.690, 124.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.780 - 1.750
R-factor	0.1963
Rwork	0.195
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 7FAB & 8FAB
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.780	42.780	1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.066	0.026	1.291
Rmeas	0.072	0.029	1.424
Total number of observations	347976
Number of reflections	52582	689	3667
<I/σ(I)>	14.17	48.88	0.88
Completeness [%]	99.1	98.6	94.8
Redundancy	6.618	6.09	5.568
CC(1/2)	0.999	0.999	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.085 M HEPES, pH 7.5, 17% PEG4000, 8.5% isopropanol, 15% glycerol