6WYR

Crystal structure of anti-Muscle Specific Kinase (MuSK) Fab, MuSK1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	65.870, 71.720, 106.180
Unit cell angles	90.00, 104.37, 90.00

Refinement procedure

Resolution	49.040 - 1.800
R-factor	0.1812
Rwork	0.180
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dw2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.040	49.040	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.073	0.021	1.089
Rmeas	0.086	0.025	1.304
Total number of observations	306753
Number of reflections	88524	1048	6403
<I/σ(I)>	9.68	40.19	0.87
Completeness [%]	99.5	98.1	98.5
Redundancy	3.465	3.442	3.238
CC(1/2)	0.998	0.999	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	20% PEG6000, 0.1 M bicine, pH 8.5