6WYB
RTX (Reverse Transcription Xenopolymerase) in complex with an RNA/DNA hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 68.970, 112.940, 148.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.500 |
| R-factor | 0.2674 |
| Rwork | 0.267 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k8z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 40901 | 2997 |
| <I/σ(I)> | 10.88 | 2.1 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.9 | 5 |
| CC(1/2) | 0.996 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1 M sodium cacodylate, pH 6.5, 2 M ammonium sulfate, 0.2 M sodium chloride |
