6WYA
RTX (Reverse Transcription Xenopolymerase) in complex with a DNA duplex and dAMPNPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.960, 96.360, 112.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.780 - 2.410 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k8z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 39853 | 2913 |
| <I/σ(I)> | 12.02 | 2.06 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.6 |
| CC(1/2) | 0.998 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2 M potassium acetate, 20% PEG3350 |
