6WY1
Crystal structure of an engineered thermostable dengue virus 2 envelope protein dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2019-11-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 125.800, 125.800, 142.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.670 - 3.420 |
| R-factor | 0.3187 |
| Rwork | 0.318 |
| R-free | 0.33660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oan |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.481 |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.580 | 3.690 |
| High resolution limit [Å] | 3.420 | 3.420 |
| Rpim | 0.162 | 1.264 |
| Number of reflections | 9507 | 1901 |
| <I/σ(I)> | 9.2 | 2.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 16.1 | 16.2 |
| CC(1/2) | 0.982 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 296 | 15% PEG 3350 pH 4.4, 0.1M Sodium Acetate Trihydrate + 0.1M Sodium Iodide |
