6WX9
SOX2 bound to Importin-alpha 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-02-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.460, 61.420, 69.100 |
| Unit cell angles | 90.00, 95.49, 90.00 |
Refinement procedure
| Resolution | 29.050 - 2.800 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b18 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.661 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 16476 | 2377 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 98.8 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.994 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M sodium/potassium phosphate, 20% (w/v) PEG3350 |
