6WW9

Crystal structure of human REV7(R124A)-SHLD3(35-58) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	75.723, 75.723, 220.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.040 - 2.700
R-factor	0.2163
Rwork	0.215
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.002
RMSD bond angle	0.529
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.040	2.796
High resolution limit [Å]	2.700	2.700
Rmerge	0.165	1.319
Rmeas	0.188	1.497
Rpim	0.087	0.684
Number of reflections	18113	1755
<I/σ(I)>	8.87	1.42
Completeness [%]	97.8	99.26
Redundancy	4.3	4.3
CC(1/2)	0.991	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.2 M ammonium acetate, 0.1 M Na citrate pH 5.6, 30% PEG4000