6WW4
Crystal structure of HERC2 ZZ domain in complex with histone H3 tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-11-29 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.28 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 24.933, 76.439, 33.012 |
Unit cell angles | 90.00, 102.52, 90.00 |
Refinement procedure
Resolution | 38.219 - 2.252 |
R-factor | 0.2032 |
Rwork | 0.199 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ds6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.850 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
Rmerge | 0.083 | 0.061 | 0.157 |
Rmeas | 0.091 | 0.067 | 0.184 |
Rpim | 0.036 | 0.028 | 0.093 |
Total number of observations | 31141 | ||
Number of reflections | 5256 | 583 | 314 |
<I/σ(I)> | 13.6 | ||
Completeness [%] | 91.0 | 97.3 | 54.7 |
Redundancy | 5.9 | 5.9 | 3.2 |
CC(1/2) | 0.994 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, and 17% PEG 10000 |