6WVG

human CD53

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	49.310, 209.770, 73.280
Unit cell angles	90.00, 100.29, 90.00

Refinement procedure

Resolution	35.610 - 2.900
R-factor	0.2183
Rwork	0.216
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.128	0.027	0.738
Rmeas	0.158	0.033	0.912
Rpim	0.090	0.019	0.528
Total number of observations	84126
Number of reflections	30225	1508	1548
<I/σ(I)>	6.7
Completeness [%]	93.3	90.5	97.2
Redundancy	2.8	2.8	2.8
CC(1/2)		0.998	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	295	30% PEG 400, 0.1 M KH2PO4, 0.1 M MES pH 6.0