6WUS
Crystal structure of PRL-1 phosphatase C104D mutant in complex with the Bateman domain of CNNM2 magnesium transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9768 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.138, 127.935, 152.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.063 - 2.758 |
| R-factor | 0.2426 |
| Rwork | 0.240 |
| R-free | 0.28090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mmz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 12756 | 648 |
| <I/σ(I)> | 24.6 | 1.6 |
| Completeness [%] | 94.1 | 88.96 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 294 | 0.1 M sodium acetate, pH 5.3, 2.0 M sodium formate |
