6WUR
Crystal structure of PRL-2 phosphatase C101D mutant in complex with the Bateman domain of CNNM3 magnesium transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6235 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.857, 124.544, 153.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.173 - 2.882 |
| R-factor | 0.2407 |
| Rwork | 0.238 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k22 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Number of reflections | 11721 | 1010 |
| <I/σ(I)> | 13 | 1.2 |
| Completeness [%] | 92.4 | 80.93 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 294 | 0.1 M sodium acetate, pH 5.1, 0.54 M sodium citrate |
