6WUK

Crystal structure of mouse DXO in complex with CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.862, 87.665, 54.023
Unit cell angles	90.00, 112.24, 90.00

Refinement procedure

Resolution	50.005 - 1.599
R-factor	0.1856
Rwork	0.185
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j7l
RMSD bond length	0.012
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.005	50.005	1.640
High resolution limit [Å]	1.599	7.150	1.600
Rmerge	0.047	0.023	0.757
Rmeas	0.055	0.026	0.884
Number of reflections	56513	663	4081
<I/σ(I)>	13.73	40.6	1.56
Completeness [%]	99.4	98.8	97.4
Redundancy	3.771	3.748	3.675
CC(1/2)	0.999	0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	23% (w/v) PEG3350