6WUG
Crystal Structure of S. pombe Rai1 in complex with 3'-FADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.594, 59.978, 72.545 |
| Unit cell angles | 90.00, 104.67, 90.00 |
Refinement procedure
| Resolution | 48.660 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fqg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.395 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.663 |
| Number of reflections | 32480 | |
| <I/σ(I)> | 11.84 | 1.39 |
| Completeness [%] | 94.4 | 95 |
| Redundancy | 2.011 | 2.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% (w/v) PEG 3350 and 0.2M MgCl2 |
