6WUG

Crystal Structure of S. pombe Rai1 in complex with 3'-FADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	100.594, 59.978, 72.545
Unit cell angles	90.00, 104.67, 90.00

Refinement procedure

Resolution	48.660 - 1.900
R-factor	0.193
Rwork	0.191
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fqg
RMSD bond length	0.011
RMSD bond angle	1.395
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.660	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.043	0.663
Number of reflections	32480
<I/σ(I)>	11.84	1.39
Completeness [%]	94.4	95
Redundancy	2.011	2.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% (w/v) PEG 3350 and 0.2M MgCl2