6WUF

Crystal structure of mouse DXO in complex with 3'-FADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.956, 87.877, 53.289
Unit cell angles	90.00, 113.45, 90.00

Refinement procedure

Resolution	48.890 - 1.600
R-factor	0.2011
Rwork	0.200
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j7l
RMSD bond length	0.006
RMSD bond angle	0.898
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.890	48.890	1.640
High resolution limit [Å]	1.600	7.170	1.600
Rmerge	0.076	0.035	0.476
Rmeas	0.096	0.045	0.612
Total number of observations	244274
Number of reflections	54771	1121	7496
<I/σ(I)>	10.6	27.91	1.9
Completeness [%]	94.9	91.6	93.4
Redundancy	2.351	2.488	2.234
CC(1/2)	0.995	0.996	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	23% (w/v) PEG3350