6WTY

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 253245

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	70.559, 78.189, 312.080
Unit cell angles	90.00, 94.16, 90.00

Refinement procedure

Resolution	48.701 - 3.481
R-factor	0.2666
Rwork	0.262
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.795	48.795	3.690
High resolution limit [Å]	3.480	10.240	3.480
Rmerge	0.772	0.125	2.856
Rmeas	0.857	0.139	3.179
Total number of observations	234345
Number of reflections	43497	1809	6551
<I/σ(I)>	2.54	10.9	0.53
Completeness [%]	98.7	98.5	93.1
Redundancy	5.388	5.132	5.059
CC(1/2)	0.850	0.987	0.131

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	0.2 M sodium dihydrogen phosphate pH 4.0, 16% (w/v) PEG 3,350