6WTV
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 258259
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 93.800, 98.654, 103.094 |
Unit cell angles | 114.15, 105.00, 89.69 |
Refinement procedure
Resolution | 45.006 - 3.050 |
R-factor | 0.2373 |
Rwork | 0.235 |
R-free | 0.27670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w53 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.166 | 49.166 | 3.230 |
High resolution limit [Å] | 3.050 | 9.000 | 3.050 |
Rmerge | 0.178 | 0.058 | 0.516 |
Rmeas | 0.226 | 0.073 | 0.655 |
Total number of observations | 151296 | ||
Number of reflections | 60526 | 2318 | 9524 |
<I/σ(I)> | 5.16 | 14.87 | 1.97 |
Completeness [%] | 97.4 | 95.9 | 94.9 |
Redundancy | 2.5 | 2.596 | 2.469 |
CC(1/2) | 0.971 | 0.993 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.4 M ammonium nitrate, 18% (w/v) PEG 3,350 |