6WTL

Structure of Human pir-miRNA-19b-2 Apical Loop and One-base-pair Fused to the YdaO Riboswitch Scaffold

Replaces:  6N5L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	115.340, 115.340, 115.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.491 - 2.850
R-factor	0.1898
Rwork	0.187
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
RMSD bond length	0.003
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.491	2.920
High resolution limit [Å]	2.850	2.850
Rmeas	0.061	1.620
Rpim	0.014	0.358
Number of reflections	21107	1386
<I/σ(I)>	32.8	2
Completeness [%]	100.0	99.8
Redundancy	19.8	17.9
CC(1/2)	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	297	1.7 M (NH4)2SO4, 0.2 M LI2SO4, AND 0.1 M HEPES PH 7.1