6WTG

SdeA DUB Domain in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	31.257, 43.208, 195.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.190 - 2.630
R-factor	0.2059
Rwork	0.204
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cra
RMSD bond length	0.002
RMSD bond angle	0.457
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.620	2.620
Rpim	0.082	0.398
Number of reflections	8314	394
<I/σ(I)>	10.6	2.7
Completeness [%]	99.9	100
Redundancy	5.9	5.9
CC(1/2)	0.987	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0.1 M bicine pH 9.0, 65% (v/v) MPD