6WT6
Structure of a metazoan TIR-STING receptor from C. gigas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 136.424, 136.424, 40.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.050 - 2.410 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.24160 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.568 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.050 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.094 | 0.691 |
| Number of reflections | 15377 | 1562 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 16 | |
| CC(1/2) | 0.992 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M MgCl2, 0.1 M Tris pH 8.5, and 16% PEG-4000 |
