6WT4
Structure of a bacterial STING receptor from Flavobacteriaceae sp. in complex with 3',3'-cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-08-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.848, 121.848, 48.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 1.780 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.23030 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.632 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.790 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rpim | 0.019 | 0.531 |
| Number of reflections | 34093 | 1916 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.8 | |
| CC(1/2) | 0.999 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 M ammonium sulfate, 0.2 M sodium acetate pH 4.5 |
