6WRP

Crystal Structure of PI3-E12 Fab, An Antibody Against Human Parainfluenza Virus Type III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-16
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	51.031, 79.924, 99.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.790 - 2.080
R-factor	0.1988
Rwork	0.196
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.002
RMSD bond angle	0.511
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.130
High resolution limit [Å]	2.080	2.090
Number of reflections	24469	2026
<I/σ(I)>	16.01
Completeness [%]	98.0
Redundancy	6.5
CC(1/2)	0.997	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2.2M Ammonium Sulfate, 2.2% PEG 400, 0.11M Hepes pH 7.5