6WRO
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 1-PHOSPHATASE INPP1 IN COMPLEX GADOLINIUM BUT NO LITHIUM AT 3 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 296 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-08-10 |
| Detector | UCSD MARK II |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 41 |
| Unit cell lengths | 51.640, 51.640, 143.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.280 - 3.000 |
| R-factor | 0.19534 |
| Rwork | 0.193 |
| R-free | 0.23590 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1inp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.817 |
| Data reduction software | UCSD-system |
| Data scaling software | UCSD-system |
| Phasing software | SQUASH |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.300 | 3.230 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 12719 | 1000 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 78.6 | 70 |
| Redundancy | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 293 | 10% PEG 8000, 80mM Bis-Tris, pH 5.25, 2mM Gd2(SO4)3 |
