6WRE

Crystal structure of mouse DXO in complex with 5'-OH RNA substrate mimic and calcium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	50.182, 87.795, 53.880
Unit cell angles	90.00, 112.35, 90.00

Refinement procedure

Resolution	49.830 - 2.000
R-factor	0.1896
Rwork	0.187
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.830	49.830	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.053	0.023	0.639
Rmeas	0.072	0.031	0.856
Total number of observations	110559
Number of reflections	29081	642	3864
<I/σ(I)>	8.91	24.75	1.91
Completeness [%]	96.0	97.1	91.2
Redundancy	1.99	2.084	1.953
CC(1/2)	0.997	0.998	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% (w/v) PEG 3350