6WQW

Thermobacillus composti GH10 xylanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.4586
Spacegroup name	P 43 21 2
Unit cell lengths	81.264, 81.264, 92.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.819 - 2.102
R-factor	0.2364
Rwork	0.235
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n82
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.820	2.180
High resolution limit [Å]	2.102	2.102
Number of reflections	18595	1809
<I/σ(I)>	18.26	2.23
Completeness [%]	98.4	99.78
Redundancy	22.7	19.4
CC(1/2)	1.000	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 70% (v/v) 2-Methyl-2,4-pentanediol (MPD)