6WQV
GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.610, 83.170, 85.210 |
| Unit cell angles | 93.98, 93.36, 89.27 |
Refinement procedure
| Resolution | 36.890 - 1.450 |
| R-factor | 0.1512 |
| Rwork | 0.151 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wqp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.890 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.046 | 0.433 |
| Rmeas | 0.053 | 0.509 |
| Rpim | 0.026 | 0.262 |
| Number of reflections | 224235 | 16603 |
| <I/σ(I)> | 17 | 2.79 |
| Completeness [%] | 93.2 | 68.84 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% PEG3350, BisTris Propane pH 7.0 and 200 mM Sodium Nitrate, 1 mM cellotriose, cryoprotected in above supplemented with 15% ethylene glycol. |
