6WQD
The 1.95 A Crystal Structure of the Co-factor Complex of NSP7 and the C-terminal Domain of NSP8 from SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-23 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.261, 55.103, 84.397 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 41.120 - 1.950 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wiq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.850 | 1.950 |
| Rmerge | 0.188 | 1.150 |
| Number of reflections | 30923 | 1220 |
| <I/σ(I)> | 22.5 | 1.1 |
| Completeness [%] | 97.3 | 77.9 |
| Redundancy | 8.7 | 2.2 |
| CC(1/2) | 0.962 | 0.456 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M magnesium chloride, 0.1 M Tris, pH 8.5, 20% w/v PEG8000 |
