6WQC
Crystal structure of VipF from Legionella hackeliae in complex with CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 172.062, 36.659, 59.803 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.340 |
R-factor | 0.1849 |
Rwork | 0.182 |
R-free | 0.23320 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 2.104 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.095 | 0.689 |
Rpim | 0.028 | 0.248 |
Number of reflections | 15984 | 660 |
<I/σ(I)> | 24.76 | 2 |
Completeness [%] | 95.0 | |
Redundancy | 11.6 | |
CC(1/2) | 0.993 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1 M ammonium citrate pH 7.0, 0.1 M Bis-Tris pH 7, 0.2 M ammonium acetate, Cryo: paratone |